Spinning Disc Microscopy

Spinning disk confocal microscopy primarily relies on light rejection, making it essential to capture as many emitted photons as possible. This approach allows for reduced light intensity, minimizing the risk of photobleaching and photodamage to samples. Consequently, high camera sensitivity (high QE and low noise) is crucial for effective imaging

Combined with this is a need for reasonable speed for observation of live cells (>25 fps full frame) and in some cases higher speeds (100-200 fps full frame) for more dynamic observations

Finally there is a consideration of pixel size, matching to Nyquist is normally observed, using 6.5-micron pixels in most applications but 11 or 16-micron where working with ultra-low signals at 100x often yields better results.